Application Notes: Western blot, 0.25-0.5µg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat Immunofluorescence, 5 µg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3µg/1x106 cells, Mouse ELISA, 0.1-0.5µg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500µg/ml
Flow Cytometry analysis of RAW264.7 cells using anti-Tspan-8/Tspan8 antibody. Overlay histogram showing RAW264.7 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tspan-8/Tspan8 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of Tspan-8/Tspan8 using anti-Tspan-8/Tspan8 antibody. Tspan-8/Tspan8 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Tspan-8/Tspan8 Antibody overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of Tspan-8/Tspan8 using anti-Tspan-8/Tspan8 antibody. Tspan-8/Tspan8 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Tspan-8/Tspan8 Antibody overnight at 4C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Tspan-8/Tspan8 using anti-Tspan-8/Tspan8 antibody. Tspan-8/Tspan8 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Tspan-8/Tspan8 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Tspan-8/Tspan8 using anti-Tspan-8/Tspan8 antibody. Tspan-8/Tspan8 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Tspan-8/Tspan8 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Tspan-8/Tspan8 using anti-Tspan-8/Tspan8 antibody. Tspan-8/Tspan8 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Tspan-8/Tspan8 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Tspan-8/Tspan8 using anti-Tspan-8/Tspan8 antibody. Tspan-8/Tspan8 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed
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