HighFidelity ORANGE PCR Labeling Testkit

Produktübersicht

• Artikelname: HighFidelity ORANGE PCR Labeling Testkit
• Artikelnummer: BYT-ORB692242
• Hersteller Artikelnummer: orb692242
• Alternativnummer: BYT-ORB692242-1
• Hersteller: Biorbyt
• Kategorie: Molekularbiologie

Produktbeschreibung

HighFidelity ORANGE PCR Labeling Testkit is designed to produce randomly AF594-, ATTO594-and TexasRed-modified DNA probes by PCR to find the optimal label for the orange emission wavelength range. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. All labeled-dUTP are efficiently incorporated into DNA as sub-stitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50%/25% labeled-dUTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of labeled-dUTP/dTTP ratio however, can easily be achieved with the single nucleotide format.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Spectroscopic Propertie: AF594: lambdaexc 590 nm, lambdaem 617 nm, epsilon 92.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), ATTO594: lambdaexc 602 nm, lambdaem 626 nm, epsilon 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), TexasRed: lambdaexc 583 nm, lambdaem 603 nm, epsilon 112.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).