HighFidelity RED PCR Labeling Testkit

Produktübersicht

• Artikelname: HighFidelity RED PCR Labeling Testkit
• Artikelnummer: BYT-ORB692243
• Hersteller Artikelnummer: orb692243
• Alternativnummer: BYT-ORB692243-1
• Hersteller: Biorbyt
• Kategorie: Molekularbiologie

Produktbeschreibung

HighFidelity RED PCR Labeling Testkit is designed to produce randomly Cy5-, AF647-and ATTO643-modified DNA probes by PCR to find the optimal label for the red emission wavelength range. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. All labeled-dUTP are efficiently incorporated into DNA as sub-stitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50% labeled-dUTP substi-tution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of labeled-dUTP/dTTP ra-tio however, can easily be achieved with the single nucleotide format.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Spectroscopic Propertie: Cy5: lambdaexc 649 nm, lambdaem 670 nm, epsilon 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), AF647: lambdaexc 648 nm, lambdaem 671 nm, epsilon 270.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), ATTO643: lambdaexc 643 nm, lambdaem 665 nm, epsilon 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).