Phospho-TAK1 (Thr187) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Phospho-TAK1 (Thr187) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB7049
• Hersteller Artikelnummer: orb7049
• Alternativnummer: BYT-ORB7049-100,BYT-ORB7049-200,BYT-ORB7049-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human TAK1 around the phosphorylation site of Thr187 HM(p-T)NN
• Konjugation: Unconjugated
• Alternative Synonym: TAK1(phospho T187), TAK1(phospho Thr187), TAK1(phospho T187), MAP3K7, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1, MAP3K 7, MAPKKK7, Mitogen activated protein kinase kinase kinase 7, TAK1, TGF beta activated kinase 1, TGF1a, Transforming growth factor beta activated kinase 1, M3K7_HUMAN, Map3k7, MEKK7, TGF-beta-activated kinase 1, TGF1a, Transforming growth factor-beta-activated kinase 1.

Produktbeschreibung

Phospho-TAK1 (Thr187) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 67 kDa
• Pubmed: 41369875
• UniProt: O43318
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: MAP3K7

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-Phospho-TAK1 (Thr187) antibody (orb7049), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 20 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-TAK1 (Thr187)) Polyclonal Antibody, Unconjugated (orb7049) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: A431 (Human) Cell Lysate at 30 ug, Lane 4: 293T (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-TAK1 (Thr187) (orb7049) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 75 kD.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-TAK1 (Thr187) (orb7049) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 67 kD, Observed band size: 70 kD.

IHC-P analysis of Human stomach using Phospho-TAK1 Thr187 Polyclonal Antibody at 1:400 dilution.

IHC-P analysis of Mouse brain using Phospho-TAK1 Thr187 Polyclonal Antibody at 1:400 dilution.