Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Formulierung:
Lyophilized
Target-Kategorie:
Cd155
Application Verdünnung:
Western blot, 0.25-0.5µg/ml, Mouse, Rat ELISA, 0.1-0.5µg/ml, -
Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Poliovirus Receptor/PVR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 60-80 KD. The expected band size for Poliovirus Receptor/PVR is at 60-80 KD.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
