Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Formulierung:
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Target-Kategorie:
GZMB
Application Verdünnung:
Western Blot (2-4ug/ml),,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 C followed by cooling at RT for 20 min
Anwendungsbeschreibung:
Application Notes: Positive Control: A431 cells. Human tonsil, spleen or Hodgkins Lymphoma tissue (IHC)
Analysis of Protein Array containing >19000 full-length human proteins using Granzyme B Monospecific Mouse Monoclonal Antibody (GZMB/3014) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDAs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDAs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Formalin-fixed, paraffin-embedded human spleen stained with Biotinylated Granzyme B Mouse Monoclonal Antibody (GZMB/3014).
Formalin-fixed, paraffin-embedded human Spleen stained with Granzyme B Monospecific Mouse Monoclonal Antibody (GZMB/3014).
Formalin-fixed, paraffin-embedded human Tonsil stained with Granzyme B Monospecific Mouse Monoclonal Antibody (GZMB/3014).
SDS-PAGE Analysis Purified Granzyme B Mouse Monoclonal Antibody (GZMB/3014). Confirmation of Integrity and Purity of Antibody.
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