Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Formulierung:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Target-Kategorie:
CD40LG
Application Verdünnung:
ELISA (For coating, order antibody without BSA), Flow Cytometry (2-4ug/million cells), ,Immunofluorescence (2-4ug/ml), Western Blot (2-4ug/ml),,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requi
Anwendungsbeschreibung:
Application Notes: Positive Control: Jurkat cells. Tonsil, Spleen or Thymus
Analysis of Protein Array containing more than 19000 full-length human proteins using CD40-Ligand Mouse Monoclonal Antibody (CD40LG/2761) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDAs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDAs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Flow Cytometric Analysis of Jurkat cells using CD40L-Monospecific Mouse Monoclonal Antibody (CD40LG/2761) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescent staining of paraformaldehyde-fixed Jurkat cells using CD40L Mouse Monoclonal Antibody (CD40LG/2761) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
SDS-PAGE Analysis Purified CD40L Mouse Monoclonal Antibody (CD40LG/2761). Confirmation of Purity and Integrity of Antibody.
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