Human N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

Artikelnummer: BYT-ORB775077
Artikelname: Human N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
Artikelnummer: BYT-ORB775077
Hersteller Artikelnummer: orb775077
Alternativnummer: BYT-ORB775077-48, BYT-ORB775077-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: NT-Pro-BNP ELISA Kit, ELISA Kit, N-BNP ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP).
Konzentration: 2500 pg/mL
Detektionsbereich: 39.07-2500 pg/mL
Sensitivitaet: 15 pg/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: standard: 2500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve