PPIB ELISA Kit, PPI-B ELISA Kit, CYP-S1 ELISA Kit, CyP-B ELISA Kit, SCYLP ELISA Kit, PPIase B ELISA Kit, Rotamase B ELISA Kit, S-cyclophilin ELISA Kit, Peptidylprolyl Isomerase B ELISA Kit, Peptidyl-Prolyl Cis-Trans Isomerase B ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin B(CYPB).
Application Notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYPB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CYPB in the samples is then determined by comparing the OD of the samples to the standard curve
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