IGHG2 ELISA Kit, Ig Gamma-2 Chain C Region ELISA Kit, Immunoglobulin Heavy Constant Gamma 2 ELISA Kit, G2m Marker ELISA Kit, Immunoglobulin Gm2 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G2(IgG2).
Application Notes: standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human IgG2 in the samples is then determined by comparing the OD of the samples to the standard curve
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