Rabbit Insulin Like Growth Factor 2 (IGF2) ELISA Kit

Artikelnummer: BYT-ORB776305
Artikelname: Rabbit Insulin Like Growth Factor 2 (IGF2) ELISA Kit
Artikelnummer: BYT-ORB776305
Hersteller Artikelnummer: orb776305
Alternativnummer: BYT-ORB776305-48, BYT-ORB776305-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Rabbit
Alternative Synonym: INSIGF ELISA Kit, IGF-II ELISA Kit, Somatomedin A ELISA Kit, Preptin ELISA Kit, Insulin-like growth factor II Ala-25 Del ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2(IGF2).
Konzentration: 300 ng/mL
Detektionsbereich: 4.69-300 ng/mL
Sensitivitaet: 1.9 ng/mL
Proben: Serum, plasma and other biological fluids.
Anwendungsbeschreibung: Application Notes: standard: 300 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve