Mouse Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit

Produktübersicht

• Artikelname: Mouse Tubulin Polymerization Promoting Protein (TPPP) ELISA Kit
• Artikelnummer: BYT-ORB776509
• Hersteller Artikelnummer: orb776509
• Alternativnummer: BYT-ORB776509-48, BYT-ORB776509-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Mouse
• Alternative Synonym: TPPP1 ELISA Kit, p25alpha ELISA Kit, p25 ELISA Kit, TPPP/p25 ELISA Kit, Brain Specific Protein p25 Alpha ELISA Kit, 25 kDa brain-specific protein ELISA Kit

Produktbeschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Polymerization Promoting Protein(TPPP).

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.051 ng/mL
• UniProt: Q7TQD2
• Proben: Tissue homogenates and other biological fluids.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TPPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TPPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse TPPP in the samples is then determined by comparing the OD of the samples to the standard curve