Human Pappalysin 2 (PAPPA2) ELISA Kit

Artikelnummer: BYT-ORB776898
Artikelname: Human Pappalysin 2 (PAPPA2) ELISA Kit
Artikelnummer: BYT-ORB776898
Hersteller Artikelnummer: orb776898
Alternativnummer: BYT-ORB776898-48, BYT-ORB776898-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PAPP-A2 ELISA Kit, PAPPE ELISA Kit, PLAC3 ELISA Kit, Placenta-Specific 3 ELISA Kit, Pregnancy-associated plasma protein A2 ELISA Kit, Pregnancy-associated plasma protein E1 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pappalysin 2(PAPPA2).
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.059 ng/mL
UniProt: Q9BXP8
Proben: Serum, plasma and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAPPA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAPPA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PAPPA2 in the samples is then determined by comparing the OD of the samples to the standard curve
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