Human Bone Morphogenetic Protein 10 (BMP10) ELISA Kit

Artikelnummer: BYT-ORB777057
Artikelname: Human Bone Morphogenetic Protein 10 (BMP10) ELISA Kit
Artikelnummer: BYT-ORB777057
Hersteller Artikelnummer: orb777057
Alternativnummer: BYT-ORB777057-48, BYT-ORB777057-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: BMP-10, Bmp10, BMP10
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 10(BMP10).
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.064 ng/mL
UniProt: O95393
Proben: Serum, plasma, tissue homogenates and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human BMP10 in the samples is then determined by comparing the OD of the samples to the standard curve
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