Human Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit

Produktübersicht

• Artikelname: Human Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit
• Artikelnummer: BYT-ORB780058
• Hersteller Artikelnummer: orb780058
• Alternativnummer: BYT-ORB780058-48, BYT-ORB780058-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: WNT-12 ELISA Kit, SHFM6 ELISA Kit

Produktbeschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 10B(WNT10B).

Produkteigenschaften

• Konzentration: 4000 pg/mL
• Detektionsbereich: 62.5-4000 pg/mL
• Sensitivitaet: 27.5 pg/mL
• UniProt: O00744
• Proben: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT10B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT10B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT10B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human WNT10B in the samples is then determined by comparing the OD of the samples to the standard curve