Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) ELISA Kit

Produktübersicht

• Artikelname: Human Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) ELISA Kit
• Artikelnummer: BYT-ORB780735
• Hersteller Artikelnummer: orb780735
• Alternativnummer: BYT-ORB780735-48, BYT-ORB780735-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: Mal ELISA Kit, Wyatt ELISA Kit, MyD88 Adapter-Like ELISA Kit, Adaptor protein Wyatt ELISA Kit

Produktbeschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Interleukin 1 Receptor Domain Containing Adaptor Protein(TIRAP).

Produkteigenschaften

• Konzentration: 20 ng/mL
• Detektionsbereich: 0.32-20 ng/mL
• Sensitivitaet: 0.109 ng/mL
• UniProt: P58753
• Proben: Tissue homogenates and other biological fluids.

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIRAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIRAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TIRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TIRAP in the samples is then determined by comparing the OD of the samples to the standard curve