This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gamma-Endorphin(gEP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gamma-Endorphin(gEP).
Konzentration:
1000 pg/mL
Detektionsbereich:
15.63-1000 pg/mL
Sensitivitaet:
4.8 pg/mL
Proben:
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse gEP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse gEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse gEP in the samples is then determined by comparing the OD of the samples to the standard curve
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