Human Cancerous Inhibitor of PP2A (CIP2A) ELISA Kit
Artikelnummer:
BYT-ORB780931
Hersteller Artikelnummer:
orb780931
Alternativnummer:
BYT-ORB780931-48, BYT-ORB780931-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Applikation:
ELISA
Spezies Reaktivität:
Human
Alternative Synonym:
p90 ELISA Kit, KIAA1524 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cancerous Inhibitor Of PP2A(CIP2A).
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CIP2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CIP2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CIP2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CIP2A in the samples is then determined by comparing the OD of the samples to the standard curve
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