Rabbit A Disintegrin and Metalloproteinase with Thrombospondin 5 (ADAMTS5) ELISA Kit

Produktübersicht

• Artikelname: Rabbit A Disintegrin and Metalloproteinase with Thrombospondin 5 (ADAMTS5) ELISA Kit
• Artikelnummer: BYT-ORB781460
• Hersteller Artikelnummer: orb781460
• Alternativnummer: BYT-ORB781460-48, BYT-ORB781460-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Rabbit
• Alternative Synonym: ADMP-2 ELISA Kit, Aggrecanase-2 ELISA Kit

Produktbeschreibung

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloproteinase With Thrombospondin 5(ADAMTS5).

Produkteigenschaften

• Konzentration: 10 ng/mL
• Detektionsbereich: 0.16-10 ng/mL
• Sensitivitaet: 0.061 ng/mL
• Proben: Tissue homogenates, cell lysates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ADAMTS5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ADAMTS5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ADAMTS5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit ADAMTS5 in the samples is then determined by comparing the OD of the samples to the standard curve