Mouse Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit
Artikelnummer:
BYT-ORB781536
Hersteller Artikelnummer:
orb781536
Alternativnummer:
BYT-ORB781536-48, BYT-ORB781536-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Applikation:
ELISA
Spezies Reaktivität:
Mouse
Alternative Synonym:
BSP1 ELISA Kit, JV4-1 ELISA Kit, JV41 ELISA Kit, MADH1 ELISA Kit, MADR1 ELISA Kit, SMAD Family Member 1 ELISA Kit, Mad-related protein 1 ELISA Kit, Transforming growth factor-beta-signaling protein 1 ELISA Kit, Mothers against DPP homolog 1 ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 1(Smad1).
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse Smad1 in the samples is then determined by comparing the OD of the samples to the standard curve
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten