General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit

Produktübersicht

• Artikelname: General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit
• Artikelnummer: BYT-ORB781857
• Hersteller Artikelnummer: orb781857
• Alternativnummer: BYT-ORB781857-48, BYT-ORB781857-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: All
• Alternative Synonym: NAADP

Produktbeschreibung

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nicotinic Acid Adenine Dinucleotide Phosphate(NAADP).

Produkteigenschaften

• Konzentration: 10000 ng/mL
• Detektionsbereich: 156.25-10000 ng/mL
• Sensitivitaet: 51 ng/mL
• Proben: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 10000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve