The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with Mannose Binding Lectin(MBL) protein.
Konzentration:
400 ng/mL
Detektionsbereich:
6.25-400 ng/mL
Sensitivitaet:
2.54 ng/mL
Proben:
Serum, plasma and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve
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