This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Diacylglycerol(DAG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Diacylglycerol(DAG).
Konzentration:
100000 pg/mL
Detektionsbereich:
1562.5-100000 pg/mL
Sensitivitaet:
143 pg/mL
Proben:
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 100000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DAG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of DAG in the samples is then determined by comparing the OD of the samples to the standard curve
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