General Phosphatidylinositol Trisphosphate (PIP3) ELISA Kit

Produktübersicht

• Artikelname: General Phosphatidylinositol Trisphosphate (PIP3) ELISA Kit
• Artikelnummer: BYT-ORB782079
• Hersteller Artikelnummer: orb782079
• Alternativnummer: BYT-ORB782079-48, BYT-ORB782079-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: All
• Alternative Synonym: PtdIns(3,4,5)P3 ELISA Kit

Produktbeschreibung

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Phosphatidylinositol Trisphosphate(PIP3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylinositol Trisphosphate(PIP3).

Produkteigenschaften

• Konzentration: 500 ng/mL
• Detektionsbereich: 7.82-500 ng/mL
• Sensitivitaet: 2.84 ng/mL
• Proben: serum, plasma and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 500 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve