Human Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II) ELISA Kit
Artikelnummer:
BYT-ORB782364
Hersteller Artikelnummer:
orb782364
Alternativnummer:
BYT-ORB782364-48, BYT-ORB782364-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Applikation:
ELISA
Spezies Reaktivität:
Human
Alternative Synonym:
PIVKA-II ELISA Kit, PIVKA II ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIVKA-II(Protein Induced Vitamin K Absence or Antagonist-II).
Konzentration:
20 ng/mL
Detektionsbereich:
0.32-20 ng/mL
Sensitivitaet:
0.129 ng/mL
Proben:
serum, plasma and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-II. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIVKA-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIVKA-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIVKA-II in the samples is then determined by comparing the OD of the samples to the standard curve
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