Epididymis Protein 4 ELISA Kit, dJ461P17.6 ELISA Kit, EDDM4 ELISA Kit, epididymal protein 4 ELISA Kit, Epididymal secretory protein E4 ELISA Kit, Epididymis specific whey acidic protein type four disulfide core ELISA Kit, HE 4 ELISA Kit, Major epididymis specific protein E4 ELISA Kit, Major epididymis-specific protein E4 ELISA Kit, MGC57529 ELISA Kit, Putative protease inhibitor WAP5 ELISA Kit, WAP 5 ELISA Kit, WAP domain containing protein HE4 ELISA Kit, WAP domain containing protein HE4 V4 ELISA Kit, WAP four disulfide core domain 2 ELISA Kit, WAP four disulfide core domain protein 2 ELISA Kit, WAP four-disulfide core domain protein 2 ELISA Kit, WAP5 ELISA Kit, WFDC 2 ELISA Kit, WFDC2 ELISA Kit, WFDC2_HUMAN ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HE4(Epididymal protein 4).
Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HE4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human HE4 in the samples is then determined by comparing the OD of the samples to the standard curve
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