The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CA 27-29(cancer antigen 27-29).
Konzentration:
100 U/mL
Detektionsbereich:
1.57-100 U/mL
Sensitivitaet:
0.8 U/mL
Proben:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Anwendungsbeschreibung:
Application Notes: standard: 100 U/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA 27-29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA 27-29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA 27-29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human CA 27-29 in the samples is then determined by comparing the OD of the samples to the standard curve
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