Human Type IIA Collagen N-Propeptide (PIIANP) ELISA Kit

Artikelnummer: BYT-ORB782603
Artikelname: Human Type IIA Collagen N-Propeptide (PIIANP) ELISA Kit
Artikelnummer: BYT-ORB782603
Hersteller Artikelnummer: orb782603
Alternativnummer: BYT-ORB782603-48, BYT-ORB782603-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: PIIANP ELISA Kit
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Type IIA Collagen N-Propeptide(PIIANP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Type IIA Collagen N-Propeptide(PIIANP).
Konzentration: 1000 ng/mL
Detektionsbereich: 15.6-1000 ng/mL
Sensitivitaet: 5.9 ng/mL
Proben: Serum, plasma, other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIANP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIIANP in the samples is then determined by comparing the OD of the samples to the standard curve
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