Rat Arginine Vasopressin (AVP) ELISA Kit

Artikelnummer: BYT-ORB782678
Artikelname: Rat Arginine Vasopressin (AVP) ELISA Kit
Artikelnummer: BYT-ORB782678
Hersteller Artikelnummer: orb782678
Alternativnummer: BYT-ORB782678-48, BYT-ORB782678-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Rat
Alternative Synonym: ARVP ELISA Kit, AVP ELISA Kit, VP ELISA Kit, Arginine Vasopressin ELISA Kit, Arg-Vasopressin ELISA Kit, Antidiuretic Hormone ELISA Kit, Diabetes Insipidus ELISA Kit, Neurohypophyseal ELISA Kit, Argipressin ELISA Kit, Vasopressin ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin (AVP).
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.116 ng/mL
UniProt: P01186
Proben: Tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AVP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AVP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AVP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat AVP in the samples is then determined by comparing the OD of the samples to the standard curve
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