Rat Translocator Protein (TSPO) ELISA Kit

Artikelnummer: BYT-ORB782698
Artikelname: Rat Translocator Protein (TSPO) ELISA Kit
Artikelnummer: BYT-ORB782698
Hersteller Artikelnummer: orb782698
Alternativnummer: BYT-ORB782698-48, BYT-ORB782698-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Rat
Alternative Synonym: IBP ELISA Kit, PKBS ELISA Kit, PBR ELISA Kit, pk18 ELISA Kit, mDRC ELISA Kit, MBR ELISA Kit, BZRP ELISA Kit, DBI ELISA Kit, PTBR ELISA Kit, Mitochondrial benzodiazepine receptor ELISA Kit, Peripheral-Type Benzodiazepine Receptor/Recognition Site ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocator Protein(TSPO).
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.124 ng/mL
UniProt: P16257
Proben: tissue homogenates, cell lysates and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat TSPO in the samples is then determined by comparing the OD of the samples to the standard curve
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