Native Porcine Carboxypeptidase B, CAS [[9025-24-5]]

Produktübersicht

• Artikelname: Native Porcine Carboxypeptidase B, CAS [[9025-24-5]]
• Artikelnummer: CRM-NATE-0152
• Hersteller Artikelnummer: NATE-0152
• Alternativnummer: CRM-NATE-0152-20
• Hersteller: Creative BioMart
• Kategorie: Biochemikalien

Produktbeschreibung

Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.

Produkteigenschaften

• Konzentration: ~50 units per mg protein
• CAS Nummer: [9025-24-5]

Anwendungsbeschreibung

• Anwendungsbeschreibung: Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis. The enzyme from Creative Enzymes has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine (s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.