Phosphorylase b is used to study the conversion mechanism of inactive phosphorylase b to active phosphorylase in muscle. Phosphorylase b is used to study which factors influence the conversion of phosphorylase b to phosphorylase a such as temperature, AMP, fluoride and detergents. It is used to study phosphorylase b deficiency mutations. The enzyme from Creative Enzymes has been used in the calibration of Sepharose C1-6B columns while studying the molecular weight of methylamine dehydrogenase subunits. It has been used in ion mobility-mass spectrometry studies of phosphorylase B ions that have been generated with supercharging reagents, in charge-reducing buffer. It has also been used for the preparation of p32 labeled phosphorylase A using phosphorylase kinase and [32P]ATP.
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