ABCB1 Monoclonal Antibody, IgG2b, Clone: [5C12D3], Unconjugated, Mouse

Produktübersicht

• Artikelname: ABCB1 Monoclonal Antibody, IgG2b, Clone: [5C12D3], Unconjugated, Mouse
• Artikelnummer: CSB-MA001046A0M
• Hersteller Artikelnummer: CSB-MA001046A0m
• Alternativnummer: CSB-MA001046A0M-100UL, CSB-MA001046A0M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: ABC20 antibody, ABCB1 antibody, ATP binding cassette, sub family B (MDR/TAP), member 1 antibody, ATP-binding cassette sub-family B member 1 antibody, CD243 antibody, CLCS antibody, Colchicin sensitivity antibody, Doxorubicin resistance antibody, GP170 antibody, MDR1 antibody, MDR1_HUMAN antibody, Multidrug resistance 1 antibody, Multidrug resistance protein 1 antibody, P glycoprotein 1 antibody, P gp antibody, P-glycoprotein 1 antibody, PGY1 antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [5C12D3]
• Isotyp: IgG2b
• UniProt: P08183
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein A purified
• Formulierung: Liquid
• Target-Kategorie: ABCB1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Western Blot
Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate
All lanes: ABCB1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 141 kDa
Observed band size: 141-250 KDa
Exposure time:5min