CD19 Monoclonal Antibody, IgG2b, Clone: [4C6G1], Unconjugated, Mouse

Produktübersicht

• Artikelname: CD19 Monoclonal Antibody, IgG2b, Clone: [4C6G1], Unconjugated, Mouse
• Artikelnummer: CSB-MA004888A1M
• Hersteller Artikelnummer: CSB-MA004888A1m
• Alternativnummer: CSB-MA004888A1M-100UL, CSB-MA004888A1M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19, CD19

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [4C6G1]
• Isotyp: IgG2b
• UniProt: P15391
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein A purified
• Formulierung: Liquid
• Target-Kategorie: CD19

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200

Bilder

Overlay histogram showing Raji cells stained with CSB-MA004888A1m (red line) at 1:50. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of CSB-MA004888A1m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA004888A1m diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: CD19 antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 61 kDa
Observed band size: 95 kDa