CD9 Monoclonal Antibody, IgG2a, Clone: [5A6D8], Unconjugated, Mouse
Artikelnummer:
CSB-MA004969A0M
- Bilder (7)
| Artikelname: | CD9 Monoclonal Antibody, IgG2a, Clone: [5A6D8], Unconjugated, Mouse |
| Artikelnummer: | CSB-MA004969A0M |
| Hersteller Artikelnummer: | CSB-MA004969A0m |
| Alternativnummer: | CSB-MA004969A0M-100UL, CSB-MA004969A0M-50UL |
| Hersteller: | Cusabio |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Tetraspanin 29 antibody, 5H9 antibody, 5H9 antigen antibody, Antigen defined by monoclonal antibody 602 29 antibody, Antigen defined by monoclonal antibody 60229 antibody, BA-2/p24 antigen antibody, BA2 antibody, BTCC 1 antibody, BTCC1 antibody, CD9 antibody, CD9 antigen antibody, CD9 antigen p24 antibody, CD9 molecule antibody, CD9_HUMAN antibody, Cell growth inhibiting gene 2 protein antibody, Cell growth-inhibiting gene 2 protein antibody, DRAP 27 antibody, DRAP27 antibody, GIG2 antibody, Growth inhibiting gene 2 protein antibody, Leukocyte antigen MIC3 antibody, MIC3 antibody, Motility related protein antibody, Motility-related protein antibody, MRP 1 antibody, MRP-1 antibody, MRP1 antibody, p24 antibody, p24 antigen antibody, Tetraspanin-29 antibody, Tspan 29 antibody, Tspan-29 antibody, TSPAN29 antibody |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [5A6D8] |
| Isotyp: | IgG2a |
| UniProt: | P21926 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | CD9 |
| Application Verdünnung: | Recommended dilution: WB: 1:2000-1:16000, IHC: 1:50-1:200 |
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IHC image of MA004969A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of MA004969A0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate All lanes CD9 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa Exposure time:5min |
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Western Blot Positive WB detected in: A549 whole cell lysate at 20ug, 10ug, 5ug, 2.5ugAll lanes: CD9 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa Exposure time:10min |
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Western Blot Positive WB detected in: 20ug A549 whole cell lysate CD9 antibody at 1:2000, 1:4000, 1:8000, 1:16000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa Exposure time:10min |
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Western Blot Positive WB detected in: 1. Exosomes extracted from plasma 2. Exosomes extracted from serum 3. Exosomes extracted from urine 4. Exosomes extracted in cell supernatant 5. Exosomes extracted from latex 6. Exosomes extracted from saliva 7. Hela whole cell lysate All lanes CD9 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa Exposure time:5min |
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Western Blot Positive WB detected in: 1. Exosomes extracted from saliva 2. Hela whole cell lysate All lanes Ywhaz antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa Exposure time:5min |
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