CTLA4 Monoclonal Antibody, IgG1, Clone: [9D2E9], Unconjugated, Mouse

Produktübersicht

• Artikelname: CTLA4 Monoclonal Antibody, IgG1, Clone: [9D2E9], Unconjugated, Mouse
• Artikelnummer: CSB-MA006163A0M
• Hersteller Artikelnummer: CSB-MA006163A0m
• Alternativnummer: CSB-MA006163A0M-100UL, CSB-MA006163A0M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: ALPS5 antibody, CD 152 antibody, CD antibody, CD152 antibody, CD152 antigen antibody, CD152 isoform antibody, Celiac disease 3 antibody, CELIAC3 antibody, CTLA 4 antibody, CTLA-4 antibody, CTLA4 antibody, CTLA4_HUMAN antibody, Cytotoxic T cell associated 4 antibody, Cytotoxic T lymphocyte antigen 4 antibody, Cytotoxic T lymphocyte associated 4 antibody, Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody, Cytotoxic T lymphocyte associated antigen 4 antibody, Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody, Cytotoxic T lymphocyte associated protein 4 antibody, Cytotoxic T lymphocyte associated serine esterase 4 antibody, Cytotoxic T lymphocyte protein 4 antibody, Cytotoxic T-lymphocyte protein 4 antibody, Cytotoxic T-lymphocyte-associated antigen 4 antibody, GRD4 antibody, GSE antibody, ICOS antibody, IDDM12 antibody, insulin-dependent diabetes mellitus 12 antibody, Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody, OTTHUMP00000216623 antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [9D2E9]
• Isotyp: IgG1
• UniProt: P16410
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: CTLA4

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB: 1:1000-1:5000, IHC: 1:200-1:1000

Bilder

IHC image of CSB-MA006163A0m diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-MA006163A0m diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-MA006163A0m diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: CEM whole cell lysate, K562 whole cell lysate, Raji whole cell lysate
All lanes: CTLA4 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25, 7, 9, 20 KDa
Observed band size: 34 KDa
Exposure time:5min