Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C.
Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
Immunoprecipitating lacZ in 293T transfected whole cell lysateLane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysateLane 2: CSB-MA009476A0m (2µl) + 293T transfected whole cell lysate (500µg)Lane 3: 293T transfected whole cell lysate (20µg)For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
Western BlotPositive WB detected in Recombinant proteinAll lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 117 kDaObserved band size: 130 kDa
Western BlotPositive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)All lanes: lacZ antibody at 1:2000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 117 kDaObserved band size: 130 kDa
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