PRDX3 Monoclonal Antibody, IgG1, Clone: [6E6E12], Unconjugated, Mouse

Produktübersicht

• Artikelname: PRDX3 Monoclonal Antibody, IgG1, Clone: [6E6E12], Unconjugated, Mouse
• Artikelnummer: CSB-MA018656A1M
• Hersteller Artikelnummer: CSB-MA018656A1m
• Alternativnummer: CSB-MA018656A1M-100UL, CSB-MA018656A1M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Antioxidant protein 1 antibody, AOP 1 antibody, AOP-1 antibody, AOP1 antibody, HBC189 antibody, MER5 antibody, MGC104387 antibody, MGC24293 antibody, mitochondrial antibody, peroxiredoxin 3 antibody, Peroxiredoxin III antibody, Peroxiredoxin-3 antibody, PRDX3 antibody, PRDX3_HUMAN antibody, PRO1748 antibody, Protein MER5 homolog antibody, PRX III antibody, Prx-III antibody, PRX3 antibody, SP 22 antibody, SP-22 antibody, SP22 antibody, Thioredoxin dependent peroxide reductase mitochondrial antibody, Thioredoxin-dependent peroxide reductase antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [6E6E12]
• Isotyp: IgG1
• UniProt: P30048
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: PRDX3

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing Hela cells stained with CSB-MA018656A1m (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Overlay Peak curve showing MCF7 cells stained with CSB-MA018656A1m (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cells with(CSB-MA018656A1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of MCF7 cells with(CSB-MA018656A1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate
All lanes: PRDX3 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 KDa
Exposure time:5min