NFE2L2 Monoclonal Antibody, IgG2b, Clone: [2F6C6], Unconjugated, Mouse
Artikelnummer:
CSB-MA614961A0M
- Bilder (7)
| Artikelname: | NFE2L2 Monoclonal Antibody, IgG2b, Clone: [2F6C6], Unconjugated, Mouse |
| Artikelnummer: | CSB-MA614961A0M |
| Hersteller Artikelnummer: | CSB-MA614961A0m |
| Alternativnummer: | CSB-MA614961A0M-100UL, CSB-MA614961A0M-50UL |
| Hersteller: | Cusabio |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | erythroid derived 2 antibody, HEBP1 antibody, like 2 antibody, NF E2 related factor 2 antibody, NF-E2-related factor 2 antibody, NF2L2_HUMAN antibody, NFE2 related factor 2 antibody, NFE2-related factor 2 antibody, Nfe2l2 antibody, Nrf 2 antibody, NRF2 antibody, Nuclear factor (erythroid derived 2) like 2 antibody, Nuclear factor antibody, nuclear factor erythroid 2 like 2 antibody, Nuclear factor erythroid 2 related factor 2 antibody, Nuclear factor erythroid 2-related factor 2 antibody, Nuclear factor erythroid derived 2 like 2 antibody |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [2F6C6] |
| Isotyp: | IgG2b |
| UniProt: | Q16236 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | NFE2L2 |
| Application Verdünnung: | Recommended dilution: WB: 1:1000-1: 8000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
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Overlay Peak curve showing Hela cells stained with CSB-MA614961A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Overlay Peak curve showing HepG2 cells stained with CSB-MA614961A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of NIH/3T3 cells with CSB-MA614961A0m at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). |
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Immunofluorescence staining of HepG2 cells with CSB-MA614961A0m at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). |
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IHC image of CSB-MA614961A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-MA614961A0m diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-mouse IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: NFE2L2 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: RAW264.7 whole cell lysate Lane 6: K562 whole cell lysate Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 68 KDa Observed band size: 68-100 KDa Exposure time: 1min |
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