SMURF1 Monoclonal Antibody, IgG2a, Clone: [1A9H4], Unconjugated, Mouse

Produktübersicht

• Artikelname: SMURF1 Monoclonal Antibody, IgG2a, Clone: [1A9H4], Unconjugated, Mouse
• Artikelnummer: CSB-MA875720A0M
• Hersteller Artikelnummer: CSB-MA875720A0m
• Alternativnummer: CSB-MA875720A0M-100UL, CSB-MA875720A0M-50UL
• Hersteller: Cusabio
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: E3 ubiquitin-protein ligase SMURF1 antibody, hSMURF1 antibody, KIAA1625 antibody, Smad specific E3 ubiquitin ligase 1 antibody, SMAD specific E3 ubiquitin protein ligase 1 antibody, Smad ubiquitination regulatory factor 1 antibody, SMAD-specific E3 ubiquitin-protein ligase 1 antibody, SMUF1_HUMAN antibody, SMURF 1 antibody, smurf1 antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [1A9H4]
• Isotyp: IgG2a
• UniProt: Q9HCE7
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: SMURF1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Bilder

Overlay Peak curve showing Hela cells stained with CSB-MA875720A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cells with CSB-MA875720A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of HepG2 cells with CSB-MA875720A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

IHC image of CSB-MA875720A0m diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA875720A0m diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate
All lanes: SMURF1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 KDa
Exposure time:5min