BCL3 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: BCL3 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA002623LA01HU
• Hersteller Artikelnummer: CSB-PA002623LA01HU
• Alternativnummer: CSB-PA002623LA01HU-100UG, CSB-PA002623LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: B cell CLL/lymphoma 3 antibody, B cell leukemia/lymphoma 3 antibody, B cell lymphoma 3 encoded protein antibody, B-cell lymphoma 3 protein antibody, Bcl 3 antibody, BCL 4 antibody, BCL-3 antibody, Bcl3 antibody, BCL3_HUMAN antibody, BCL4 antibody, Chronic lymphatic leukemia protein antibody, D19S37 antibody, Protein Bcl 3 antibody, Proto-oncogene BCL3 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P20749
• Puffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: BCL3

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Bilder

IHC

IHC image of CSB-PA002623LA01HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Immunofluorescence staining of Hela cell with CSB-PA002623LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with CSB-PA002623LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: BCL3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa