CSNK2A1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CSNK2A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA006072LA01HU
• Hersteller Artikelnummer: CSB-PA006072LA01HU
• Alternativnummer: CSB-PA006072LA01HU-100UG, CSB-PA006072LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: Casein kinase 2 alpha 1 polypeptide antibody, Casein kinase II alpha 1 antibody, Casein kinase II alpha 1 subunit antibody, Casein kinase II alpha subunit antibody, Casein kinase II subunit alpha antibody, CK II alpha antibody, CK II antibody, CK2 alpha antibody, CK2 catalytic subunit alpha antibody, CK2A1 antibody, CKII antibody, CKIIalpha antibody, CSK21_HUMAN antibody, CSNK2A1 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P68400
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: CSNK2A1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescent analysis of HepG2 cells using CSB-PA006072LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IHC image of CSB-PA006072LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA006072LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate
All lanes: CSNK2A1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 30 kDa
Observed band size: 46 kDa

Immunoprecipitating CSNK2A1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA006072LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA006072LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)