HIST1H3A (Ab-36) Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HIST1H3A (Ab-36) Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA010418OA36NME3HU
• Hersteller Artikelnummer: CSB-PA010418OA36nme3HU
• Alternativnummer: CSB-PA010418OA36NME3HU-100UL, CSB-PA010418OA36NME3HU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: H3 histone family member E pseudogene antibody, H3 histone family, member A antibody, H3/A antibody, H31_HUMAN antibody, H3F3 antibody, H3FA antibody, Hist1h3a antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST3H3 antibody, histone 1, H3a antibody, Histone cluster 1, H3a antibody, Histone H3 3 pseudogene antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P68431
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: HIST1H3A

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200

Bilder

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H3A (CSB-PA010418OA36nme3HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunofluorescent analysis of Hela cells using CSB-PA010418OA36nme3HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010418OA36nme3HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010418OA36nme3HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela cell acid extracts, Jurkat cell acid extracts, HEK293 cell acid extracts, Rat liver tissue, Rat kidney tissue, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue
All lanes: HIST1H3A antibody at 1.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa