HIST1H4A (Ab-5) Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HIST1H4A (Ab-5) Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA010429OA05NCRHU
• Hersteller Artikelnummer: CSB-PA010429OA05ncrHU
• Alternativnummer: CSB-PA010429OA05NCRHU-100UL, CSB-PA010429OA05NCRHU-50UL
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Rat
• Konjugation: Unconjugated
• Alternative Synonym: dJ160A22.1 antibody, dJ160A22.2 antibody, dJ221C16.1 antibody, dJ221C16.9 antibody, FO108 antibody, H4 antibody, H4.k antibody, H4/a antibody, H4/b antibody, H4/c antibody, H4/d antibody, H4/e antibody, H4/g antibody, H4/h antibody, H4/I antibody, H4/j antibody, H4/k antibody, H4/m antibody, H4/n antibody, H4/p antibody, H4_HUMAN antibody, H4F2 antibody, H4F2iii antibody, H4F2iv antibody, H4FA antibody, H4FB antibody, H4FC antibody, H4FD antibody, H4FE antibody, H4FG antibody, H4FH antibody, H4FI antibody, H4FJ antibody, H4FK antibody, H4FM antibody, H4FN antibody, H4M antibody, HIST1H4A antibody, HIST1H4B antibody, HIST1H4C antibody, HIST1H4D antibody, HIST1H4E antibody, HIST1H4F antibody, HIST1H4H antibody, HIST1H4I antibody, HIST1H4J antibody, HIST1H4K antibody, HIST1H4L antibody, HIST2H4 antibody, HIST2H4A antibody, Hist4h4 antibody, Histone 1 H4a antibody, Histone 1 H4b antibody, Histone 1 H4c antibody, Histone 1 H4d antibody, Histone 1 H4e antibody, Histone 1 H4f antibody, Histone 1 H4h antibody, Histone 1 H4i antibody, Histone 1 H4j antibody, Histone 1 H4k antibody, Histone 1 H4l antibody, Histone 2 H4a antibody, histone 4 H4 antibody, Histone H4 antibody, MGC24116 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P62805
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: Antigen Affinity Purified
• Formulierung: Liquid
• Target-Kategorie: HIST1H4A

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:100-1:1000, IHC:1:20-1:200, IF:1:1-1:10, IP:1:200-1:2000

Bilder

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CSB-PA010429OA05ncrHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunofluorescence staining of Hela cells with CSB-PA010429OA05ncrHU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA010429OA05ncrHU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA010429OA05ncrHU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Rat spleen tissue
All lanes: HIST1H4A antibody at 0.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa

Immunoprecipitating HIST1H4A in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010429OA05ncrHU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010429OA05ncrHU (5µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)