HTRA2 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HTRA2 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA010902EA01HU
• Hersteller Artikelnummer: CSB-PA010902EA01HU
• Alternativnummer: CSB-PA010902EA01HU-100UG, CSB-PA010902EA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: High temperature requirement protein A2 antibody, HTRA 2 antibody, HtrA like serine protease antibody, HtrA serine peptidase 2 antibody, HtrA, E. coli, homolog of, 2 antibody, HtrA2 antibody, HTRA2_HUMAN antibody, mitochondrial antibody, Omi stress regulated endoprotease antibody, Omi stress-regulated endoprotease antibody, PARK 13 antibody, PARK13 antibody, Protease serine 25 antibody, PRSS 25 antibody, PRSS25 antibody, Serine protease 25 antibody, Serine protease HTRA2 antibody, Serine protease HTRA2 mitochondrial antibody, Serine protease htra2 mitochondrial precursor antibody, Serine protease omi antibody, Serine proteinase OMI antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O43464
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: HTRA2

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

Bilder

Immunoprecipitating HTRA2 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA010902EA01HU in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010902EA01HU (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (20µg)

IHC image of CSB-PA010902EA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA010902EA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA010902EA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, HT29 whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate
All lanes: HTRA2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 49, 47, 40 kDa
Observed band size: 39 kDa