ELP1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: ELP1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA011571LA01HU
• Hersteller Artikelnummer: CSB-PA011571LA01HU
• Alternativnummer: CSB-PA011571LA01HU-100UG, CSB-PA011571LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: DKFZp781H1425 antibody, DYS antibody, Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody, Elongator complex protein 1 antibody, ELP 1 antibody, ELP1 antibody, ELP1_HUMAN antibody, FD antibody, FLJ12497 antibody, IKAP antibody, IkappaB kinase complex associated protein antibody, IkappaB kinase complex-associated protein antibody, ikbkap antibody, IKI 3 antibody, IKI3 antibody, IKK complex associated protein antibody, IKK complex-associated protein antibody, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody, OTTHUMP00000063889 antibody, p150 antibody, TOT 1 antibody, TOT1 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O95163
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: ELP1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescence staining of Hela cells with CSB-PA011571LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
All lanes: IKBKAP antibody at 5.3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 151 kDa
Observed band size: 151 kDa

IHC image of CSB-PA011571LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA011571LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating IKBKAP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA011571LA01HU in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA011571LA01HU (6ug) + Hela whole cell lysate (1mg)
Lane 3: Hela whole cell lysate (20ug)