GDI1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: GDI1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA01229A0RB
• Hersteller Artikelnummer: CSB-PA01229A0Rb
• Alternativnummer: CSB-PA01229A0RB-100UG, CSB-PA01229A0RB-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: 1A antibody, FLJ41411 antibody, GDI-1 antibody, gdi1 antibody, GDIA_HUMAN antibody, GDIL antibody, GDP dissociation inhibitor 1 antibody, Guanosine diphosphate dissociation inhibitor 1 antibody, Mental retardation X-linked 41 antibody, Mental retardation X-linked 48 antibody, MRX41 antibody, MRX48 antibody, Oligophrenin 2 antibody, Oligophrenin-2 antibody, OPHN2 antibody, Protein XAP 4 antibody, Protein XAP-4 antibody, Protein XAP4 antibody, Rab GDI alpha antibody, Rab GDP dissociation inhibitor alpha antibody, RABGD1A antibody, RABGDIA antibody, XAP 4 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P31150
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: GDI1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Bilder

IHC image of CSB-PA01229A0Rb diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA01229A0Rb diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: GDI1 antibody at 6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa

Immunofluorescence staining of MCF-7 cells with CSB-PA01229A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).