KRT7 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: KRT7 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA012564LA01HU
• Hersteller Artikelnummer: CSB-PA012564LA01HU
• Alternativnummer: CSB-PA012564LA01HU-100UG, CSB-PA012564LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: CK 7 antibody, CK-7 antibody, CK7 antibody, Cytokeratin 7 antibody, Cytokeratin-7 antibody, D15Wsu77e antibody, K2C7 antibody, K2C7_HUMAN antibody, K7 antibody, Keratin 7 antibody, Keratin 7, type II antibody, Keratin type II cytoskeletal 7 antibody, Keratin, 55K type II cytoskeletal antibody, Keratin, simple epithelial antibody, Keratin, simple epithelial type I, K7 antibody, Keratin, type II cytoskeletal 7 antibody, Keratin-7 antibody, Krt2-7 antibody, KRT7 antibody, MGC11625 antibody, MGC129731 antibody, MGC3625 antibody, Sarcolectin antibody, SCL antibody, Type II mesothelial keratin K7 antibody, Type-II keratin Kb7 antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P08729
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: KRT7

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescent analysis of HepG2 cells using CSB-PA012564LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA012564LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA012564LA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: KRT7 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Immunoprecipitating KRT7 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of ,CSB-PA012564LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA012564LA01HU (8µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)