LATS1 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: LATS1 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA012769LA01HU
• Hersteller Artikelnummer: CSB-PA012769LA01HU
• Alternativnummer: CSB-PA012769LA01HU-100UG, CSB-PA012769LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: h-warts antibody, Large tumor suppressor homolog 1 antibody, LATS large tumor suppressor homolog 1 antibody, LATS1 antibody, LATS1_HUMAN antibody, Serine threonine protein kinase LATS1 antibody, Serine/threonine-protein kinase LATS1 antibody, WARTS antibody, WARTS protein kinase antibody, wts antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O95835
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: LATS1

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: IHC:1:100-1:1200, IF:1:50-1:500, IP:1:200-1:2000

Bilder

Immunofluorescent analysis of Hela cells using CSB-PA012769LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA012769LA01HU at dilution of 1:100

IHC image of CSB-PA012769LA01HU diluted at 1:1200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA012769LA01HU diluted at 1:1200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating LATS1 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA012769LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA012769LA01HU (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)