PVALB Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PVALB Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA019092HA01HU
• Hersteller Artikelnummer: CSB-PA019092HA01HU
• Alternativnummer: CSB-PA019092HA01HU-100UG, CSB-PA019092HA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Konjugation: Unconjugated
• Alternative Synonym: D22S749 antibody, MGC116759 antibody, Parvalbumin alpha antibody, PRVA_HUMAN antibody, PVALB antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: P20472
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: PVALB

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Bilder

Immunofluorescent analysis of HepG2 cells using CSB-PA019092HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA019092HA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Mouse brain tissue, Mouse kidney tissue
All lanes: PVALB antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa

Immunofluorescence staining of U251 cells with CSB-PA019092HA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019092HA01HU at dilution of 1:100